The role of Pseudomonas Fluorescens bacteria and Trichoderma Harzianum fungus in the interaction with poultry manure in the growth and yield of basil plants (Ocimum Basilicum L.)

This study was conducted on basil (Ocimum basilicum L.) in the spring season of 2026 in Al-Qadisiyah Governorate - Nafar. The study aimed to determine the effect of three factors: the first factor was bacterial biofertilizer at two levels (Non inoculation and inoculation with Pseudomonas fluorescens); the second factor was fungal biofertilizer (no inoculation and inoculation with Trichoderma harzianum); and the third factor was poultry manure at three levels (3, 6, and 7) t. ha -1, consisting of a single biofertilizer and a mixture of the fungus Trichoderma harzianumand the bacteria Pseudomonas fluorescens and the second factor was poultry manure (M) at three levels (2, 5, and 7) t.ha -1. The (R.C.B.D.) experimental design was used in the field factorial experiment. The results showed that single inoculations and their dual and triple combinations achieved a significant increase in the studied traits: available NPK (mg/kg), bacterial number (-610*C.F.U. g.soil-1), fungal number (-310*C.F.U. g.soil-1), and leaf number(leaves.plant-1), reaching (32, 49, 33, 65, 77, and 42)%, respectively, compared to the control. The results of the field experiment on basil confirmed that the economic costs of fertilizers can be reduced while maintaining soil health, thus enabling the use of sustainable Agriculture methods.