Isolation and identification of some pathogenic and contaminated bacterial species for burns in the city of Hilla

The objective of this study was to gather samples of burns and wounds from patients who were admitted to Al-Sadiq Hospital. A total of 25 samples of burns and wounds were obtained from individuals of diverse racial backgrounds. This study spanned from June 11, 2022, to March 30, 2023. The specimens were cultivated on several culture mediums, including Nutrient agar, Mannitol salt agar, and MacConkey agar. The bacterium Pseudomonas aeruginosa was identified and separated from other microorganisms. By culturing them on a Nutrient agar medium Upon cultivating, a distinct shift in the medium's colour was seen, transitioning from a pale yellow hue to a vibrant phosphorous green shade. This alteration serves as an indicator of the bacteria's pigment-producing capability, which is a defining trait of this pathogenic strain. Staphylococcus aureus was additionally obtained by cultivating the burn sample on a medium called Mannitol salt agar. Upon incubation, the media underwent a noticeable colour shift from pink to yellow, indicating that the bacteria successfully fermented mannitol sugar and consequently altered the pH of the medium from alkaline to acidic. Bacteria have the choice to utilise this media. Staphylococcus aureusan additional harmful bacterium, Klebsiella, was cultured on MacConkey's medium. The object was observed in a pale violet hue. This bacterium possesses the capacity to undergo lactose fermentation, making it a fundamental microbe in MacConkey's medium. The mucous texture was also used to diagnose it. Additional biochemical assays were conducted. The diagnosis of the previously isolated samples was verified with the tests for Indol, Urea, Catalase, Oxidase, and Coagulase. A test to determine the sensitivity of an antibiotic was conducted. A pseudomonas bacterium antibiotic susceptibility test was performed on the samples using a panel of seven different antibiotics. Following a 24-hour incubation period, it was determined that the sample exhibited resistance to all antibiotics employed in the test, hence indicating the bacteria's resistance.